Deoxycytidylate Deaminase EVIDENCE FORANEW ENZYME IN CELLS INFECTED BY THE VIRUS OF HERPES SIMPLEX By HILARY A. ROLTON* and HAMISH M. KEIR
نویسندگان
چکیده
The activities ofdCMP deaminase andDNA polymerase I increased twofold and fivefold in BHK-21/C13 cells after infection by the virus of herpes simplex. The increases were greatly diminished, and under certain conditions prevented, by inclusion of actinomycin D or cycloheximide in the cell-virus system during the infective cycle. ThedCMP deaminase purified from infected cells harvested 8h after infection differed from the deaminase purified from non-infected cells inasmuch as (a) it was more resistant to heating at 37°C; (b) the substrate(dCMP) concentration athalf-maximum velocitywas lower; (c)maximum activation was achieved by a lower concentration of dCTP; (d) it was more resistant to inhibition by dTTP; and (e) it behaved differently when assayed in the presence of a herpes-virus-specific antiserum. The DNA polymerase activity in the infected cells was markedly decreased in the presence of the herpes-virus-specific antiserum.
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